Buffer Na-Citrate pH 4.8

Resep buffer Na-Citrate pH 4.8

Larutan Stock buffer 1M

Citric acid monohydrate 210 g
DI water 750 mL
NaOH – tambahkan hingga mendekati pH 4.3 50 – 60 g

Larutkan semua bahan hingga terlarut semua, kemudian tambahkan hingga volumenya 1 L. Tambahkan NaOH jika perlu hingga pH buffer kurang lebih 4.5.
Pengenceran stock buffer dengan air hingga konsentrasi 50 mM, pH larutan seharusnya 4.8.

Pembuatan buffer lain: Buffer Phosphate, Acetate, Lactatate, Citrate

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